Laboratory 8: Gel Electrophoresis and Proteins

Laboratory 8: Gel Electrophoresis and Proteins

Materials:
-Procell electrophoresis apparatus
-Combs
-Gel molds
-Power units
-Buffers
-Gels
-Beads
-String

Exercises:
- Understand sickle cell anemia
- Understand what sickle cell anemia looks like
- Understand Transcription and Translation
- Understand where transcription and translation occurs
- Understand RNA, DNA, tRNA, and mRNA
- Understand the structure of a protein

Laboratory 7: Onion DNA Preparation

Laboratory 7: Onion DNA Preparation

Materials:
-onions
-beakers
-hot water bath
-blender
-cheesecloth
-pipette
-chloroform
-homogenizing solution
-erlenmeyer flask

Exercises:
-Learn what DNA looks like
-Learn to isolate DNA out of cells into a pure form
-Learn to precipitate DNA out of cells into a pure form
-Learn what homogenization is
-Learn what deproteinization is
-Learn what precipitation is

Laboratory 9: Biological Chemicals, Nutrition, and Health

Laboratory 9: Biological Chemicals, Nutrition, and Health

Materials

- Test tube racks

- Hot plate

- beaker

- text tubes

- onion juice

- potato juice

- distilled water

- Benedict's reagent 

- lugol's iodine

- Sudan III

- flour extracts

- cream

- coconut

- margarine

- filter paper

- pencils

- water bath

- dedicated Pasteur pipet

- egg white

- chicken broth

- wax pencils

- biuret's agent

- CuSO4

- instant and ground coffee

- honey

- olive oil

- peanut oil

- candy

- lite and regular pancake syrup

- gelatin

Exercises

- Test for carbohydrates, proteins, and fats in food

- Test for different chemicals

- Learn how the tests are different

- Understand how to use the computer and different software to asses the quality of your diet and favorite foods

- Find the percentage of your diet that composed of the major food groups

- Analyze food labels and figure out what a packaged food item really contains